EvaGreen® dye is a green fluorescent nucleic acid dye useful for several applications including qPCR, high-resolution DNA melt curve analysis (HRM)1, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), routine solution DNA quantification and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® dye Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life. Under standard hot-start conditions (i.e., 94?C in pH 8-9 Tris), CheetahTM Taq regains both the 3'-5' polymerase and 5'-exonuclease activities within two minutes. CheetahTM Taq is also superior to antibody-based hot-start DNA polymerases due to its complete supression of enzyme activity at room temperature and full recovery of enzyme activity following activation. CheetahTM Taq is a patent-pending invention completely independent of AmpliTaq Gold and related technologies. We welcome you to explore licensing opportunities with us.