Phenix High Fidelity Enzymes are thermostable enzymes possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. They produces blunt-ended amplicons of up to 5Kb in length are supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided. The specificity and performance of our High Fidelity Enzymes can be further improved with the use of 2x PolyMate Additive, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

Phenix Mastermix is a convenient ready-to-go 2x reaction mix designed for high fidelity and maximum experiment reproducibility. Mastermix contains Phenix High Fidelity Enzymes, MgCl2 and ultra-pure dNTPs. Mastermix is optimized and ready-to-use: the user adds only water, template and primers. Mastermix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured, by a reduction in the number of pipetting steps that can lead to pipetting errors. Mastermix is supplied with an additional 50mM of MgCl2 solution for optional optimization of reaction conditions.

Until May 31, 2010, Buy $400 of select reagents*, Get a $25 Visa Gift Card!
[Mention Code: PCR-225]
*Qualifing Reagents: TAQ, Mastermix & DNTP's-RNA Isolation-RT PCR & cDNA Synthesis
To see our Frequently Asked Questions (FAQs) for High Fidelity Enzymes & Mastermix, please click here.