New RNA Purification Kits Purify High-Quality, Intact RNA in 1 Hour
- Convenient Protocol: Quick isolation of high quality RNA
- Performs well with large or small amts of tissue or cells
- Ready to use solutions for a wide variety of samples
- Save up to 40% off pricing of similar products
- Applications include Isolation of high quality RNA from diverse biological material, including animal and plant tissues rich in polysaccharides and proteoglycans
- Storage and Shipping: Shelf Life of 6 months at +4 C Shipped on Blue Ice
Technical Details
RNA Purification Kits are a ready-to-use reagent for the isolation of total RNA from cells and tissues. RNA Purification Kits maintains the integrity of the extracted RNA, while disrupting cells and subsequently dissolving cell components. The reagent combines a blend of phenol and other components.
The biological sample is homogenized or lysed in RNA Purification Kits and then separated into organic and aqueous phases. The RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol. The isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1ml of RNA Purification Kits is sufficient to isolate Total RNA from 1 x 107 cells or 100mg of tissue.
From 1mg of tissue or 1 x 106 of cultured cells, the expected yield of RNA is:
- 2-5µg from mouse kidney (tissue)
- 5-10µg from mouse liver (tissue)
- 8-15µg from epithelial cells (cultured cells)
- 20-25µg from cell fibroblasts (cultured cells)
Until January 15, 2010, Buy $400 of select reagents*, Get a $25 Visa Gift Card!
[Mention Code: PCR-225]
*Qualifing Reagents: TAQ, Mastermix & DNTP's-RNA Isolation-RT PCR & cDNA Synthesis
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