Taq for PCR Assays (requiring Hot Start) is a heat-activated thermostable DNA polymerase isolated from a novel organism. Taq for PCR Assays provides improved specificity as compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. Taq for PCR Assays is inactive at room temperature and therefore prior to PCR cycling, requires activation by heat treatment for 10 minutes. Subsequently, the reaction can be handled according to the user´s existing protocols for thermostable DNA Polymerases.

2x Mastermix for PCR Assays (requiring Hot Start) is a complete ready-to-use heat-activated 2x reaction-mix, which simply requires the user to add only water, template and primers, and then pre-heat to 95°C for 10 minutes to successfully carry out PCR assays. The 10-minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, since the enzyme is inactive at initial low temperatures. 2x Mastermix is based on Taq for PCR Assays , which leaves an ´A´ overhang, and has been optimized for a wide variety of templates. An additional 50mM MgCl2 solution is included should any fine adjustments be required. It dramatically reduces the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to pipetting errors.

2x Red Mastermix for PCR Assays (requiring Hot Start) is a complete ready-to-use heat-activated 2x reaction-mix, which simply requires the user to add only water, template and primers, and then pre-heat to 95°C for 10 minutes to successfully carry out PCR assays. The 10 minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, since the enzyme is inactive at initial low temperatures. 2x Red Mastermix combines all of the features and advantages of ImmoMix, and contains an additional inert red dye. This non-toxic, non-hazardous red dye allows users to load samples directly onto a gel, without the need to add loading buffer since the mix is of sufficiently high density to sink to the bottom of the gel. Adequate mixing is also ensured when reactions are set up.

2x Red Mastermix is based on Taq for PCR Assays , which leaves an ´A´ overhang, and has been optimized for a wide variety of templates. An additional 50mM MgCl2 solution is included should any fine adjustments be required. It dramatically reduce the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to pipetting errors

Until May 31, 2010, Buy $400 of select reagents*, Get a $25 Visa Gift Card!
[Mention Code: PCR-225]
*Qualifing Reagents: TAQ, Mastermix & DNTP's-RNA Isolation-RT PCR & cDNA Synthesis
To see our Frequently Asked Questions (FAQs) for Taq for PCR Assays, please click here.
To see our Frequently Asked Questions (FAQs) for 2x Mastermix for PCR Assays, please click here.
To see our Frequently Asked Questions (FAQs) for 2x Red Mastermix for PCR Assays, please click here.